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This post was originally entered as part of the European Association of Cancer Research Science Communication Prize. When I am asked what I do for a living at a party or a gathering, I will often say “I am a scientist” or “I am a cancer researcher”. This is often met with “Wow that isContinue reading “The Cancer Researcher Blog post”

Colony PCR protocol Aim: To identify positive colonies from cloning experiments. I always find that colony PCR protocols can be very hit and miss, some people will say it depends on how many bacteria cells you are loading in to your PCR reaction. I think this protocol is quite simple and has two main advantages.Continue reading “A simple colony PCR protocol”

Cloning of gRNAs into pLENTICRISPRv2-GFP plasmid Aim: To generate pLENTICRISPRv2-GFP plasmids containing sgRNAs of interest for CRISPR Cas9 mediated knock out of a gene of interest. Method: For this protocol we will use the plasmid pLENTICRISPRv2-GFP This plasmid was modified to express GFP by the Feldser lab using a plasmid originally from the Feng ZhangContinue reading “Cloning a lentiviral CRISPR construct”

This site is a way to share things I have learnt along the way in my career as a cancer researcher. Also a way of keeping track of books I am reading or interesting papers I come across. Additionally, I want this to be a place that people can come to learn tips and tricksContinue reading “Introduction”